Story City is a city in Story County, Iowa, United States. Story City is located within the Ames, Iowa Metropolitan Statistical Area which envelops all of Story County and which, when held in conjunction with the Boone, Iowa Micropolitan Statistical Area, complements the larger Ames-Boone, Iowa Combined Statistical Area. The population was 3,431 in the 2010 census, a slight increase from 3,228 in the 2000 census.